چکیده مقاله
The development of targeted drug delivery systems using smart nanoparticles offers a transformative approach to cancer treatment by improving therapeutic efficacy and minimizing off target toxicity In this study, we report the design, synthesis, and evaluation of folate conjugated, pH and temperature responsive PLGA PEG nanoparticles encapsulating doxorubicin for selective cancer therapy The nanoparticles were synthesized via nanoprecipitation and characterized for particle size, morphology, zeta potential, drug encapsulation efficiency, and release kinetics under physiological and tumor mimicking conditions Targeting capability was assessed in folate receptor positive MCF 7 and negative NIH 3T3 cells using confocal microscopy and flow cytometry Cytotoxicity was measured using MTT assays, while in vivo pharmacokinetics, biodistribution, tumor suppression, and histopathology were analyzed in murine xenograft models Results showed monodisperse nanoparticles ~140 nm, negative surface charge with high drug loading and stability Folate functionalization significantly enhanced cellular uptake in MCF 7 cells compared to non targeted particles Zhang et al , 2015 Drug release was minimal at physiological pH 7 4 but accelerated at acidic pH 5 5 and elevated temperature 42°C , simulating tumor conditions Lee et al , 2005 In vivo studies revealed extended circulation time, higher tumor accumulation, and greater tumor growth inhibition with reduced systemic toxicity compared to free doxorubicin Histological analysis confirmed selective tumor apoptosis without damage to major organs This work highlights the synergistic benefit of combining active targeting and dual stimuli responsiveness in a single nanoplatform, supporting its potential for clinical translation in precision oncology The platform could be further adapted for combination therapies or integrated with diagnostic agents for theranostic applications
کلیدواژهها
نویسندگان
Mohammad Javad Sohrabi
Department of pharmaceutical, Faculty of pharmaceutical, Damghan Islamic Azad University, Damghan, Iran
Parisa Miralinaghi
Department of Medicinal chemistry, Faculty of pharmaceutical, Damghan Islamic Azad University, Damghan, Iran
Mohammad Reza Sohrabi Renani
Department of medicine, Faculty of medicine, Guilan University of Medical Sciences, Rasht, Iran
شیوه ارجاع
Sohrabi, Mohammad Javad and Miralinaghi, Parisa and Sohrabi Renani, Mohammad Reza,1404,Targeted drug delivery using smart nanoparticles,5th World Congress of new findings in Health ,Health Sciences and Educational Sciences
ارائهشده در
مجموعه مقالات پنجمین کنگره جهانی یافته های نوین در سلامت، علوم بهداشتی و علوم تربیتی31 تیر 1404