چکیده مقاله
Diabetes is a disease associated with impairment of the male reproductive system that causes complications such as decreased testosterone, the diameter of the seminiferous tubule, libido, and fertility Extracellular matrix ECM molecules are involved in testicular development and spermatogenesis Laminin and collagen are key proteins in seminiferous tubule basement membrane and play an important role in spermatogenesis The present study was conducted to investigate the effect of diabetes on collagen IV and laminin α5 changes in mice testis In this experimental study, 40 mice C57BL/6 were divided randomly into 4 groups: 1 Control group: without intervention, 2 Diabetic group: treated mice with 50 mg/kg streptozotocin STZ , 3 Diabetic Insulin group: treated mice with STZ and insulin, and 4 Sham group: received citrate buffer After 35 days, the left testes of all specimens were used for Real Time PCR while their right testes were applied for immunohistochemical study and Periodic acid–Schiff PAS staining This study showed that gene expression and immunoreactivity of laminin α5 and collagen IV were significantly increased in diabetic mice compared to other groups P
کلیدواژهها
نویسندگان
Elnaz Khordad
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mohammad Reza Nikravesh
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mehdi Jalali
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Alireza Fazel
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mojtaba Sankian
Bou Ali Research Institute, Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Fatemeh Alipour
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
شیوه ارجاع
Khordad, Elnaz and Nikravesh, Mohammad Reza and Jalali, Mehdi and Fazel, Ali Reza and Sankian, Mojtaba and Alipour, Fatemeh,1400,Diabetes up-regulated collagen IV and laminin α5 genes in mRNA and protein levels in seminiferous tubules of C57BL/6 adult mice- Running title: Correlation between diabetic and collagen IV and laminin α5,Fifth International Conference on New Findings in Midwifery, Obstetrics, Gynecology and Infertility
ارائهشده در
مجموعه مقالات پنجمین کنفرانس بین المللی یافته های نو در مامایی، زنان، زایمان و نازایی20 آبان 1400