چکیده مقاله
In Rosmarinic Acid RA is a phenolic acid which has many biological activities such as antioxidant, anti inflammatory and anti viral effects In the present study, we have studied the anti Herpes simplex type 1 HSV 1 effect of methanolic extract of Zataria multiflora which has been standardized on the basis of RA content Methanolic extract of Zatria multiflora was prepared by maceration method RA content of plant extract was measured by spectrophotometry method using the calibration curve of RA Maximum non Toxic Concentration MNTC of the plant was determined by neutral red method MNTC and lower serial dilutions of extract were examined in vitro on vero cells for their effect against HSV 1 using a plaque reduction assay Acyclovir was used as positive control Time dependent antiviral effect of Z multiflora was studied by adding the extract to HSV 1 infected vero cells at different stages of infection The percentage of RA was determined as 2 2% in Z multiflora This plant was effective in all used concentrations and significantly reduced plaque formation up to 100% at concentrations of 800 and 1000 μg mL 1 Clearly Z multiflora revealed both a time and concentration inhibition It seems that the presence of rosmarinic acid would be a determining factor for anti HSV activity of Z multiflora
نویسندگان
Mahnaz Heidarbeigi
ali mohammad arabzadehmehdi ansari dogahehfariba sharififar
ali mohammad arabzadeh
Mahnaz Heidarbeigimehdi ansari dogahehfariba sharififar
mehdi ansari dogaheh
Mahnaz Heidarbeigiali mohammad arabzadehfariba sharififar
fariba sharififar
Mahnaz Heidarbeigiali mohammad arabzadehmehdi ansari dogaheh
شیوه ارجاع
Heidarbeigi, Mahnaz and arabzadeh, ali mohammad and ansari dogaheh, mehdi and sharififar, fariba,1392,Anti Herpes simplex-1 Activity of A Standard Extract of Zataria multiflora Boiss,National Conference on the Use of Medicinal Plants in Lifestyle and Traditional Medicine,Torbat Heydarieh
ارائهشده در
مجموعه مقالات همایش ملی کاربرد گیاهان دارویی در سبک زندگی و طب سنتی6 آذر 1392 · تربت حیدریه