چکیده مقاله
Due to the valuable nature of the secondary metabolites of aquatic medicinal plants, knowing the genes involved in the expression of metabolites is one of the most important issues of these plants, and by clarifying this path, initiatives can be taken to express less or more of these genes, be special In order to discover and better understand the molecular mechanism in the lotus plant, the NPF1 gene and its expression were specifically investigated The expression of this gene has the ability to reduce Alzheimer's disease by activating neurons The sequence of this gene has higher homology with the members of the TATA box family transcription factors in the expression of cultures In this research, NPF1 gene was studied using bioinformatics sources and specific primers were designed for the amplification of this gene In this study, the characteristics of NPF1 gene in lotus plant were presented NPF1 is upregulated in mature endoles Studies of the promoter of this gene show that the transcription activation is limited to the cortical region of the roots or plant tubers Under conditions, expression of the NPF 1 knockout mutant increases stem growth and anthocyanin accumulation NPF 1 gene was used in PCR reaction The expression level of NPF 1 gene is the highest in the reproductive glands of this plant and the lowest in the flowers of this plant By analyzing the molecular structure of the transcript and based on homology modeling, its spatial shape was shown, the above structure is shown
کلیدواژهها
نویسندگان
Iman Yousefi Javan
Assistant Professor, Department of Plant Production, Faculty of Agriculture, University of TorbatHeydarieh, Torbat Heydarieh, Iran
شیوه ارجاع
Yousefi Javan, Iman,1402,Extraction And Sequencing of NPF Gene In Lotus Plant, In Order To Prevent Alzheimer's Disease,The 5th National Conference of Medicinal Plants and Traditional Medicine,Torbat Heydarieh
ارائهشده در
مجموعه مقالات پنجمین همایش ملی گیاهان دارویی و طب سنتی20 مهر 1402 · تربت حیدریه