چکیده مقاله
There is a direct correlation between asymptomatic oropharyngeal colonization by pathogenic bacteria and the prevalence of paediatric respiratory infections Streptococcus pneumoniae and Haemophilus influenzae are common cause of serious bacterial infections such as meningitis in children This study aimed to monitor healthy children to detect asymptomatic carriers of S pneumoniae and H influenzae In the present cross sectional study, real time PCR assay was developed and evaluated in comparison with culture for direct detection of S pneumoniae and H influenzae in 123 oropharynx and nasal cavity specimens from healthy children in Ilam, Iran In addition, virulence factor ply and hpd and iron uptake tbpA and piuA genes were evaluated by PCR Our results demonstrated that among all isolates only 14 S pneumoniae and eight H influenzae were identified by phenotypic methods, whereas 37 and 21 S pneumoniae and H influenza were identified, respectively, by real time PCR assay All S pneumoniae and H influenzae isolates possessed tbpA and piuA genes Also,81% 30/37 of S pneumoniae isolates were positive for ply gene, which encoded pneumolysin, as well as 90% 19/21 of H influenza isolates were positive for hpd gene Simultaneous colonization of S pneumoniae and H influenzae could indicate the importance of monitoring of healthy children to identification of carriers
کلیدواژهها
نویسندگان
A Maleki
Science Committee, Ilam University of Medical Sciences, Ilam, Iran
F Mansournia
Masters, Ilam University of Medical Sciences, Ilam, Iran
N Sadeghifard
Professor, Ilam University of Medical Sciences, Ilam, Iran
شیوه ارجاع
Maleki, A and Mansournia, F and Sadeghifard, N,1401,Rapid and direct molecular detection of Streptococcus pneumoniae and Haemophilus influenzae isolated in oropharynx and nasal cavity of children,The 11th International Conference on Health, Treatment and Health Promotion
ارائهشده در
مجموعه مقالات یازدهمین کنفرانس بین المللی بهداشت،درمان و ارتقای سلامت17 آذر 1401