چکیده مقاله
The interaction between triamcinolone acetonide TA and human growth hormone hGH was investigated using UV Vis spectrophotometry under optimized experimental conditions TA exhibited a maximum absorbance at 342 nm and followed the Beer Lambert law in the concentration range of 2 3 x 10 to 4 5 × 10 M, indicating monomeric behavior in solution Upon addition of hGH, a decrease in absorbance and the presence of an isosbestic point confirmed complex formation between TA and hGH The interaction was found to be strongly pH dependent, with maximum binding occurring at pH 2, while ionic strength showed negligible influence, suggesting an ion dipole interaction mechanism Binding stoichiometry was determined to be approximately 1:3 hGH:TA Thermodynamic parameters obtained from Van't Hoff analysis revealed negative values of ΔG°, ΔH°, and ΔS, indicating a spontaneous, exothermic, and entropy unfavorable process Temperature dependent studies showed reduced binding affinity at higher temperatures, consistent with an exothermic interaction Overall, the results demonstrate a weak but spontaneous interaction between TA and hGH, supporting the inhibitory role of TA on growth hormone activity and highlighting UV Vis spectroscopy as a simple and reliable method for biomolecular interaction studies
کلیدواژهها
نویسندگان
N. Sohrabi
Department of Chemistry, Payame Noor University, P O Box:Tehran, Iran
شیوه ارجاع
Sohrabi, N.,1404,An Integrated experimental and computational study of the interaction between triamcinolone acetonide(TA) and human growth hormone(hGH) using UV/Vis spectroscopy and Molecular Docking,Ninth International Conference on Technology Development in Chemical Engineering,Tehran
ارائهشده در
مجموعه مقالات نهمین کنفرانس بین المللی توسعه فناوری در مهندسی شیمی30 بهمن 1404 · تهران