چکیده مقاله
Tuberculosis is a common, and in many cases fatal, infectious disease This disease is caused by various species of mycobacteria, usually Mycobacterium tuberculosis This bacterium shows intrinsic and acquired resistance to all kinds of antibiotics An important mechanism of resistance to fluoroquinolones in Mycobacterium tuberculosis is the mutation in gyrA, a type II topoisomerase subunit The aim of this study is to investigate the genomic role of gyrA in mycobacterial strains In this cross sectional study, 120 isolates of Mycobacterium tuberculosis isolated from different samples, including sputum and respiratory secretions, were identified using biochemical methods Antibiotic sensitivity and resistance were determined by molecular PCR To investigate gyrA gene mutations in ciprofloxacin resistant isolates, PRRFP was performed BLAST software was used to compare gyrA gene sequence in resistant isolates The highest level of resistance to ciprofloxacin in isolates was MIC>310 μg/ml Two mutations in gyrA gene were identified in resistant isolates Also, this study showed that there is no relationship between MIC level in ciprofloxacin resistant isolates and mutation in gyrA gene These results show that the mutation in the gyrA gene is one of the most important mechanisms of resistance to ciprofloxacin in clinical isolates of Mycobacterium tuberculosis, and it seems that the mutation in gyrA, along with several genes involved in the development of resistance, has increased resistance in different isolates
کلیدواژهها
نویسندگان
Jafar Aghajani
Mycobacteriology Research Center MRC , National Research Institute of Tuberculosis and Lung Disease NRITLD , Shahid Beheshti University of Medical Sciences, Tehran, Iran
Parissa Farmia
Mycobacteriology Research Center MRC , National Research Institute of Tuberculosis and Lung Disease NRITLD , Shahid Beheshti University of Medical Sciences, Tehran, Iran
Saman Ayoubi
Mycobacteriology Research Center MRC , National Research Institute of Tuberculosis and Lung Disease NRITLD , Shahid Beheshti University of Medical Sciences, Tehran, Iran
Poopak Farmia
Mycobacteriology Research Center MRC , National Research Institute of Tuberculosis and Lung Disease NRITLD , Shahid Beheshti University of Medical Sciences, Tehran, Iran Department of Biotechnology, School of Advanced Technology in Medicine, Shahid Beh
Jalaledin Ghanavi
Mycobacteriology Research Center MRC , National Research Institute of Tuberculosis and Lung Disease NRITLD , Shahid Beheshti University of Medical Sciences, Tehran, Iran
Ali Akbar Velayati
Mycobacteriology Research Center MRC , National Research Institute of Tuberculosis and Lung Disease NRITLD , Shahid Beheshti University of Medical Sciences, Tehran, Iran
شیوه ارجاع
Aghajani, Jafar and Farmia, Parissa and Ayoubi, Saman and Farmia, Poopak and Ghanavi, Jalaledin and Velayati, Ali Akbar,1402,Examining the genomic role of gyrA in mycobacterial strains,The first international conference of health and health sciences students of Iran,Tehran
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مجموعه مقالات اولین کنفرانس بین المللی دانشجویان بهداشت و علوم سلامت ایران31 اردیبهشت 1402 · تهران