چکیده مقاله
Among different solar ultraviolet radiations only UV A completely passes through the atmosphere and reaches the ground UV A and visible spectrum of sunlight can induce photo damaging of lens proteins through endogenous photosensitizer molecules such as riboflavin RF In the current study, total soluble lens proteins TSP was incubated under direct sunlight in the presence of riboflavin as a light sensitive molecule that naturally is present in the lens Photo damaging effects of RF were evaluated by gel electrophoresis and various spectroscopic analyses SDS PAGE analysis indicated that exposure of lens soluble proteins under direct sunlight in the presence of riboflavin leads to extensive protein crosslinking and also three dimensional structural alteration of these proteins which confirmed by fluorescence spectroscopy assessments The structural alteration of protein samples was accompanied with appearance of a new absorption peak between 300 and 400 nm which may be related to formation of new chromophores Moreover, protein samples were incubated with α lipoic acid antioxidant as a preventive and therapeutic agent that naturally is present in the lens, to evaluate its protective role against photo damaging effects of RF Our results indicate that α lipoic acid can protect lens proteins against photo oxidative impairment induced by RF in a dose dependent manner Finally, the results of this study indicate that the antioxidant defense mechanism of the lens significantly contributes to protecting the lens from damaging effects of sunlight during life span
نویسندگان
Paria Dizgooni
Protein Chemistry Laboratory PCL , Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran
Kazem Khoshaman
Protein Chemistry Laboratory PCL , Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran
Reza Yousefi
Protein Chemistry Laboratory PCL , Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran
شیوه ارجاع
Dizgooni, Piria and Khoshaman, Kazem and Yousefi, Reza,1401,Protective effect of α-lipoic acid as an antioxidant compound against riboflavin-mediated photo-oxidation of TSPs (Total Soluble lens Proteins),The 11th International Conference on Health, Treatment and Health Promotion
ارائهشده در
مجموعه مقالات یازدهمین کنفرانس بین المللی بهداشت،درمان و ارتقای سلامت17 آذر 1401