چکیده مقاله
Candida albicans is the main cause of invasive fungal infections Usually, antifungal drugs such asfluconazole and amphotericin B are used to treat candidal infections But the high use of currentantifungal drugs increases drug resistance among Candida species Ergosterol is the main componentof the sterol membrane of the cell and is also responsible for maintaining the function and integrity ofthe yeast cell materials and ways :To evaluate the drug sensitivity by disc diffusion method, from standard samples and 100 clinicalisolates of Candida albicans, a cell suspension of 1 x 106 cells in one milliliter of physiological serumwas prepared and placed on Mueller Hinton agar medium containing 2% glucose and methylenesupplements Blue should be cultured at a rate of 0 5 μg/ml with a sterile swab After 25 microgramsof fluconazole discs are placed in the center of each plate after 15 minutes, and the plates areincubated at 35 degrees Celsius for 24 hours Finally, the plates were examined and measured in termsof halo diameter according to the CLSI standard using calipers findings :Studying the effect of the best drug concentration on inhibiting the production of ERG genes atdifferent MICs on the abundance of the studied genes using the polymerase chain reaction PCR method and after extracting RNA according to the manufacturer's instructions of the extraction kit anddesigning specific primers for the genes The abundance of genes was measured and the effect ofdrugs on the abundance of the desired genes was evaluated according to standard formulas Conclusion :This drug is one of the most important antifungal drugs used for both prophylaxis and treatment ofcandidiasis According to the increasing use of this antifungal, researchers believe that the use of thisdrug has increased its resistance among Candida species in the last two to three decades
کلیدواژهها
نویسندگان
Saber Noushadi
Assistant Professor, Department of Biology, Faculty of Biotechnology, Faculty of Basic Sciences,Islamic Azad University of Gachsaran
Fariba Soltani
Assistant Professor, Department of Polymer Engineering, Technical and Engineering Faculty,Shahreza Islamic Azad University
شیوه ارجاع
Noushadi, Saber and Soltani, Fariba,1402,Comparison of genes involved in fungal ergosterol (ERG) biosynthesis in Candida albicans resistant to fluconazole and amphotericin B,The 13th International Conference on Health, Treatment and Health Promotion
ارائهشده در
مجموعه مقالات سیزدهمین کنفرانس بین المللی بهداشت، درمان و ارتقای سلامت12 خرداد 1402