چکیده مقاله
Background: Crocin is a known compound with the antioxidant and anti inflammatory properties which may help to reduce the progression of neurological disorders In this study, we aimed to investigate the protective effects of crocin on beta amyloid peptide Aβ 1 40 and hydrogen peroxide H2O2 induced neurotoxicity in PC12 cells Methods: PC12 cells were pretreated with crocin and donepezil 5 and 10 μM for 2 h and then treated with Aβ 1 40 25 μM for 24 h In parallel, after pretreatment with crocin 5 and 10 μM and donepezil 5 and 10 μM for 24 h, cells were treated with H2O2 800 μM for 4 h Finally, the cell viability and intracellular reactive oxygen species ROS generation were evaluated using AlamarBlue® and 2', 7' dichlorodihydrofluorescein diacetate DCFH DA , respectively The western blot test was done to compare the protein level of phospho SAPK/JNK, SAPK/JNK, PI3 Kinase P85, Phospho PI3 Kinase P85, caspase 3 and cytochrome c cyt c Results: Crocin and donepezil could significantly decrease the Aβ toxicity and ROS level While treatment with Aβ increased Cyt c release from mitochondria to cytosol, cleaved form of caspase 3 17 kDa and activated form of SAPK/JNK p44/4 decreased the activated form of PI3 Kinase P85 protein, indicating that crocin could significantly block the apoptosis initiated with Aβ Conclusion: According to the results, crocin could be a promising candidate for further evaluations against the development of Alzheimer's disease through mitogen activated protein kinases MAPK and the phosphatidylinositol 3 kinase PI3K /protein kinase B AKT signaling PI3 K/AKT pathways
کلیدواژهها
نویسندگان
Reyhaneh Taheri
Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Zahra Tayarani Najaran
Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Elham Hadipour
Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
شیوه ارجاع
Taheri, Reyhaneh and Tayarani Najaran, Zahra and Hadipour, Elham,1402,Crocin Protects Against Beta-Amyloid Peptide-Induced Cell Apoptosis in PC12 Cells Via the PI3 K Pathway,The fourteenth international conference on health, treatment and health promotion
ارائهشده در
مجموعه مقالات چهاردهمین کنفرانس بین المللی بهداشت، درمان و ارتقای سلامت27 مرداد 1402