چکیده مقاله
Type I collagen is among the major extracellular proteins that plays a significant role in cell growth, mutility, migration, proliferation, differentiation, and inflammation. Collagen is involved in the development and progression of many tumors through various signaling pathways and cytokines. The present study investigated the effect of static magnetic field (SMF) 300 mT on the structure and molecular properties of Type I collagens. The purity of collagen was confirmed by SDS-PAGE, its secondary structure properties was revealed by Circular Dichroism (CD) and chemical bound properties by Fourier Transforms Infrared (FTIR) spectroscopy. Also, the Physico-chemical characterization of the collagen was studied using Zetasizer light Scattering and Rheological assay.Our results revealed that The pH of the treated collagen solution increased by while the Zeta potential decreased. The rheological experiments revealed changes in the viscoelastic behavior of collagen due to exposure to SMF 300mT making it less elastic. However, the secondary structure of collagen molecules did not change significantly and the typical positive and negative remained intact. Accordingly, the SMF is considered as a promising biophysical means for targeting type I collagen to control and manipulate cell metastases, motility and division as a prevention and treatment means to tackle with cancer.
کلیدواژهها
نویسندگان
Maryam Akbari
Laboratory of Membrane Biophysics and Macromolecules, Institute of Biochemistry and Biophysics, University of Tehran, Iran,
Hamid Mobasheri
Laboratory of Membrane Biophysics and Macromolecules, Institute of Biochemistry and Biophysics, University of Tehran, Iran, Institute of Biomaterials of University of Tehran and Tehran University of Medical Sciences (IBUTUMS), Tehran, Iran,
Luciana Dini
Department of Biology and Biotechnology C. Darwin, Sapienza University of Rome, Rome, Italy,
Aida mahdipour
Laboratory of Membrane Biophysics and Macromolecules, Institute of Biochemistry and Biophysics, University of Tehran, Iran,
شیوه ارجاع
Akbari, Maryam and Mobasheri, Hamid and Dini, Luciana and mahdipour, Aida,1403,Static magnetic field effects on the collagen molecules; a possible biophysical approach to control cell metastases,1st International Congress on Cancer Prevention,Zanjan
ارائهشده در
مجموعه مقالات اولین کنگره بین المللی پیشگیری از سرطان28 شهریور 1403 · زنجان