چکیده مقاله
Background & objective: Breast cancer is considered as the most prevalent type of cancer among the women with the highest mortality rate The purpose of the study was to examine the expression changes of STAT3 and AP 1 genes in MCF 7 breast adenocarcinoma cells treated with cyclophosphamide and methotrexate using RealTime PCR quantitative method Materials & Methods: These two drugs were prepared in various concentrations 1, 5 and 10 micrograms / ml and 5, 10 and 25 micrograms / ml , respectively MCF 7 cancer cells were treated with chemotherapy drugs in various groups and times after cell passage RNA extraction and cDNA synthesis were done and STAT3 and AP 1 gene expression was evaluated using RealTime PCR Results: According to the results, both genes indicated significant reductions in various times and concentrations Discussion & Conclusion: Based on the results obtained, MCF 7 cell line treatment with 2 identified drugs of STAT3 and AP 1 genes can be reduced in a dose and time dependent manner This reduction can be a proof for treatment of breast cancer
کلیدواژهها
نویسندگان
Saeid Nemati
Master of Genetics, Islamic Azad University, Zanjan Branch, Zanjan, Iran
شیوه ارجاع
Nemati, Saeid,1399,Investigating the Changes in STAT3-AP1 Gene Expression Regulated by the NF-Κb Signal Pathway in MCF-7 Cell Line of Breast Adenocarcinoma Treated With Cyclophosphamide and Methotrexate,5th International Conference on Health and Health Promotion
ارائهشده در
مجموعه مقالات پنجمین کنفرانس بین المللی بهداشت،درمان و ارتقای سلامت20 اسفند 1399