چکیده مقاله
Neonatal hypothyroidism can cause lifelong mental and physical disorders in humans Failure to diagnose early can lead to irreparable harm to infants It can be easily managed with timely detection Screening programs to diagnose hypothyroidism rely on safety methods in most countries This method is time consuming, requires laboratory equipment and must be performed by a skilled technician Therefore, ELISA is not the preferred method for diagnosing neonatal hypothyroidism and can be used as a confirmatory method in infants suspected of having neonatal hypothyroidism To design a kit, we must first obtain gold conjugated antibodies For the synthesis of nanoparticles, a concentration of 1% in 1 ml of water was added to 0 8 mg of gold nanoparticle powder HAuCL4 and heated for 3 minutes to turn pink A size analyzer was used to determine the effective diameter of the synthesized gold nanoparticles Experimentation and pairing with antibodies and evaluation of color and color intensity were evaluated in this study and particle size of 50 nm was considered Then, using SR95 1, SR95 2, and SR95 3 polyclonal antibodies, which were designed in the previous study against human TSH, along with monoclonal antibodies were used in the kit design The results of this study showed that these conjugated antibodies can be used to diagnose neonatal hypothyroidism
کلیدواژهها
نویسندگان
Neda Shakarian
Department of Clinical Biochemistry, Faculty of Medical Sciences, Dezful University of Medical Sciences, Dezful, Iran,
Maysam Mard Soltani
Department of Clinical Biochemistry, Faculty of Medical Sciences, Dezful University of Medical Sciences, Dezful, Iran,
Sima Nasri
Department of Biology, Payame Noor University, Tehran, Iran,
Mohammad Javad Rasaee
Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran,
Saeed Khalili
Department of Biology Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran,
شیوه ارجاع
Shakarian, Neda and Mard Soltani, Maysam and Nasri, Sima and Rasaee, Mohammad Javad and Khalili, Saeed,1400,Design of a diagnostic kit for anti-TSH antibody to newborn hypothyroidism,8th International Conference on Health, Treatment and Health Promotion
ارائهشده در
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