چکیده مقاله
Background & Objective: Staphylococcus aureus especially methicillin resistant S aureus MRSA strains are amajor cause of nosocomial and community acquired infections with high morbidity and mortality rates worldwide The role of Biofilm is unclear in the spread of antibiotic resistance genes among gram positive, such as S aureuswhen compared with Gram negative The aims of this study were to determine Staphylococcal adhesion genes andmulti drug resistant MDR isolates and detect the relative between their ability among clinical isolates of S aureus Methods: Sixty clinical specimens of Staphylococcus aureus were collected from clinical samples Antibioticsusceptibility of S aureus to 9 different antibiotics was investigated using disk diffusion method based on CLSI table DNA extraction of all isolates was performed by boiling method and PCR was performed Biofilm formation abilitywas investigated using microtiter method Then, by using the PCR method, the presence of the operons, icaA andicaB, icaC and icaD genes in all isolates was evaluated Also, to find out the relationship between the binding genesin the specimens and the ability to produce biofilms and the presence of icaA and icaB target genes, was investigated In the most isolated isolates of S aureus, neither genes were found simultaneously No significant correlation wasfound between the presence of fnbA binding gene and phenotypic antibiotic resistance tests P value> 0 05 Conclusion: The target genes of icaA and icaB were found in most of the isolates of this study The high presence oficaA and icaB simultaneously among a number of isolates should be considered as an important concern in theresistance phenomenon and biofilm formation
کلیدواژهها
نویسندگان
Fereshteh Shafazand
Master of science, Department of Microbiology, Faculty of Basic Sciences,Islamic Azad University, Lahijan Branch, Lahijan, Iran
Khosro Issazadeh
Assistant Professor, Department of Microbiology, Faculty of Basic Sciences,Islamic Azad University, Lahijan Branch, Lahijan, Iran
Mohammad Faezi Ghasemi
Associate Professor, Department of Microbiology, Faculty of Basic Sciences,Islamic Azad University, Lahijan Branch, Lahijan, Iran
شیوه ارجاع
Shafazand, Fereshteh and Issazadeh, Khosro and Faezi Ghasemi, Mohammad,1403,Determination of the ability to form biofilms and the presence of effectivegenes in adhesion (ica A, ica B, ica C and ica D) by Staphylococcus aureusisolated from clinical samples,18th International Conference on Health, Treatment and Health Promotion
ارائهشده در
مجموعه مقالات هجدهمین کنفرانس بین المللی بهداشت، درمان و ارتقای سلامت18 آبان 1403