چکیده مقاله
Introduction Selenium Se as an inorganic element plays a crucial role in various physiological processes within the human body, such as antioxidant defense, control of immunological response, and protection against oxidative stress Selenium nanoparticles SeNPs are gaining attention in medicine because they are easier for the body to use, less harmful than regular selenium, and have various health benefits, like fighting bacteria, acting as antioxidants, and helping to prevent cancer The synthesis method of SeNPs greatly affects their physical and chemical properties Objective This study aimed to synthesize SeNPs and determine their physicochemical properties, as well as their toxic effects on human skin cells HDF Materials and Methods SeNPs were synthesized using sodium selenite as a precursor and ascorbic acid as the reducing agent The reduction was done at ambient temperature with continuous stirring for 30 minutes The resulting solution was incubated at 37°C for 48 hours until the nanoparticle production process was completed 48 h later, dynamic light scattering DLS assessed the size and distribution of the particles, while zeta potential analysis evaluated the surface charge and stability of the solution The average particle size and zeta potential were 85 8 nm and 5 54 mV, respectively, which indicates adequate stability and a negative surface charge that may improve interaction with cell membranes Results Here, DLS and zeta potential results verify nanoparticle production SeNPs' moderate surface charge and size suggest they can penetrate biological membranes and interact with cells An MTT experiment on human dermal fibroblasts HDF assessed these SeNPs' cytotoxicity Various SeNP concentrations were applied to cells for 48 hours Concentration dependent cell viability exhibits a significant toxicity even at low concentrations The IC50 was 1/769 of the initial concentration, which suggests the toxicity of SeNPs Furthermore, flow cytometry assessment exhibited that treated cells died more through necrosis, rather than apoptosis In HDF, SeNPs may be nonspecifically harmful Conclusion Here, reduction process employing sodium selenite and ascorbic acid produced selenium nanoparticles Characterization revealed that the particles were biomedically relevant in size and surface characteristics that promote cellular absorption HDF cytotoxicity assays showed necrotic cell death and a low IC50 These findings emphasize to optimize SeNP production settings and doses for therapeutic use More research on cancer cells vs HDF and surface modifications or biofunctionalization techniques is required to increase selectivity
کلیدواژهها
نویسندگان
Mohammadreza Ashayeri
Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Mohammadmahdi Hatami
Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Amin Haratian
Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Melika Mahdoudi
Quantum Engineering and Photonics Technology Research Center at Sharif University of Technology, Tehran, Iran
Farzaneh Baniasadi
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Elaheh Amini
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
شیوه ارجاع
Ashayeri, Mohammadreza and Hatami, Mohammadmahdi and Haratian, Amin and Mahdoudi, Melika and Baniasadi, Farzaneh and Amini, Elaheh,1404,Synthesis and Cytotoxic Evaluation of Selenium Nanoparticles on Human Dermal Fibroblasts,Fourth International Conference on Leading Research of Nanotechnology Students,Tehran
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مجموعه مقالات چهارمین کنفرانس بین المللی تحقیقات پیشرو دانشجویان نانو فناوری31 شهریور 1404 · تهران