چکیده مقاله
Antimicrobial resistance in Salmonella enterica serotype Enteritidis demands new, gene targeted therapeutics We engineered a graphene oxide GO nanocarrier to deliver small interfering RNA siRNA directed against the essential 16S rRNA gene and evaluated its antibacterial efficacy in vitro GO was synthesized via a modified Hummers' method, activated with EDC/NHS, and covalently conjugated to anti 16S siRNA; physicochemical features were verified by UV Vis spectroscopy л л =230 nm; n−л ≈300 nm Efficient bacterial uptake GO siRNA was confirmed by fluorescence microscopy Functional activity was quantified by colony forming unit CFU assays and RT qPCR Relative to untreated, GO only, and siRNA only controls, GO siRNA reduced viable CFUs by >70% after 24 h one way ANOVA with Tukey's post hoc, p
کلیدواژهها
نویسندگان
Reza Safaralizadeh
Department of Animal biology, Faculty of Natural sciences, University of Tabriz, Tabriz, Iran
fereshteh fekri
Department of Genetics, Faculty of Biology, University of Rabe Rashidi, Tabriz, Iran
شیوه ارجاع
Safaralizadeh, Reza and fekri, fereshteh,1404,Targeted Suppression of Salmonella enterica Serotype Enteritidis via siRNA-Functionalized Graphene Oxide Nanocarriers Against 16S rRNA Gene,Fourth International Conference on Leading Research of Nanotechnology Students,Tehran
ارائهشده در
مجموعه مقالات چهارمین کنفرانس بین المللی تحقیقات پیشرو دانشجویان نانو فناوری31 شهریور 1404 · تهران