چکیده مقاله
In this study, a novel and sensitive UV Vis spectrophotometric method was developed for the determination of clindamycin based on its interaction with a mixed ligand system composed of Bromopyrogallol Red BPR and Methylene Blue MB The complex formation was monitored at an absorption maximum of 510 nm, showing a pronounced increase in absorbance with increasing clindamycin concentration Various spectroscopic studies, including Job's method, the molar ratio method, and Benesi Hildebrand analysis, confirmed a 1:1 stoichiometric interaction between clindamycin and the ligand system The effect of pH, temperature, and reaction time was thoroughly optimized, with the best results achieved at pH 4 5 using an acetate buffer Interference studies demonstrated the method's high selectivity against common ions, excipients, and structurally related drugs The developed method was successfully applied to pharmaceutical formulations and spiked biological samples serum and urine , with excellent recovery values 94 102% and low relative standard deviations The method is rapid, cost effective, and suitable for routine analysis in quality control and clinical laboratories
کلیدواژهها
نویسندگان
Asma mallahi
Department of Chemistry, Faculty of Basic Sciences, Payam Noor University, Tehran, Iran
شیوه ارجاع
mallahi, Asma,1404,Spectrophotometric Determination of Clindamycin Using a Mixed-Ligand System of Bromopyrogallol Red and Methylene Blue,Fourth International Conference on Leading Research of Nanotechnology Students,Tehran
ارائهشده در
مجموعه مقالات چهارمین کنفرانس بین المللی تحقیقات پیشرو دانشجویان نانو فناوری31 شهریور 1404 · تهران