چکیده مقاله
Introduction: Usually, contaminated food is one of the main causes of human infections,and poultry meat and sheep meat are important sources Enterobacter cloacae strains,having different virulence factors and multiple antibiotic resistances, are mainly consideredas an opportunistic pathogen Materials and methods: In this research, isolation of Enterobacter cloacae 384 fromchicken and sheep meat was done randomly in 1400 in Isfahan province by microbial andmolecular methods Antibiotic resistance pattern was determined by disc diffusion methodand microtitre plate method was used to investigate biofilm production The ability toproduce broad spectrum β lactamase enzymes was investigated through phenotypic andgenotypic methods Results and discussion: Out of 384 examined samples, Enterobacter cloacae wasidentified as Enterobacter cloacae in 25 samples 6 51% , which were also confirmed inthe presence of hsp60 gene in the molecular analysis Among these, 18 samples wererelated to chicken meat 72% and 7 samples 28% were related to sheep meat Among theisolates, the highest antibiotic resistance to cotrimoxazole and cefotaxime was reported in20 isolates and the lowest resistance to nitrofuranthein in 15 isolates 23 8% In microtitermethod, 15 isolates 60% showed strong biofilm reaction, 10 isolates 40% showedmoderate biofilm reaction The present study indicates that ESBL producing strains ofEnterobacter cloacae have a relatively high prevalence The increase in the amount of thesestrains is often caused by the irrational prescription of antibiotics, which requires the use ofnew antimicrobial agents to solve this problem
کلیدواژهها
نویسندگان
Fahimeh Nourbakhsh
Phd of Toxicology, Deputy of Food and Drug, Isfahan University of Medical Sciences, Isfahan,Iran
Hossein Chahardah Masoumi
Doctor of Veterinary Medicine, Deputy of Food and Drug, Isfahan University of MedicalSciences, Isfahan, Iran
Elaheh Tajbakhsh
Department of microbiology, Faculty of Basic Silences, Islamic Azad University, Shahrekord Branch,Iran
Mohammad Reza Saebi
Ph D student in Food Hygiene, Faculty of Veterinary Medicine, Islamic Azad University, ShahrekordBranch, Iran
شیوه ارجاع
Nourbakhsh, Fahimeh and Chahardah Masoumi, Hossein and Tajbakhsh, Elaheh and Saebi, Mohammad Reza,1403,Determining the pattern of antibiotic resistance in Enterobacter cloacaeisolates from meat samples in Isfahan province in 1400,Eighth International Conference on Interdisciplinary Studies in Food industry and Nutrition Science of Iran,Tehran
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مجموعه مقالات هشتمین همایش بین المللی مطالعات میان رشته ای در صنایع غذایی و علوم تغذیه ایران30 آبان 1403 · تهران