چکیده مقاله
In recent years, there has been a growing interest in sustainable and bio based resources for the production of food and pharmaceutical materials under the PharmaFood paradigm This study investigates the performance of nanostructured proteins extracted from micro nematodes Microbacterium nematodis isolated from dairy silage fermentation waste in the synthesis of novel bio polypeptides Dairy silage fermentation residues represent a rich source of organic matter and beneficial microorganisms, offering high potential for the extraction of innovative biomacromolecules Micro nematodes, as efficient biological models, are capable of degrading these wastes and producing nanostructured proteins with particle sizes ranging from 10 to 50 nm, exhibiting unique catalytic properties The primary objective of this research was to evaluate the efficacy of these proteins in synthesizing bio polypeptides with novel structures, such as peptide chains featuring enhanced disulfide bonds, and to explore their potential applications in enhancing the nutritional and therapeutic properties of dairy products and PharmaFood formulations The research methodology encompassed extraction, purification, and characterization of nanostructured proteins from nematodes cultured in silage waste media Nematodes were cultivated under controlled conditions temperature 25°C, pH 6 5 , and proteins were isolated using centrifugation and chromatography techniques The extracted proteins were characterized via dynamic light scattering DLS , transmission electron microscopy TEM , and Fourier transform infrared spectroscopy FTIR to confirm nanostructural integrity and functional groups For polypeptide synthesis, solid phase peptide synthesis SPPS was adapted with protein mediated catalysis, incorporating amino acid precursors such as L cysteine, L arginine, and L glutamine at molar ratios of 1:2:1 Reaction kinetics were monitored over 48 hours at 37°C in phosphate buffered saline PBS at pH 7 4 Synthesized polypeptides were purified by high performance liquid chromatography HPLC and analyzed for molecular weight distribution using matrix assisted laser desorption/ionization time of flight mass spectrometry MALDI TOF MS Results demonstrated that nanostructured proteins accelerated polypeptide synthesis by 3 2 fold compared to conventional enzymatic methods, yielding polypeptides with average chain lengths of 25 35 residues and molecular weights between 2 8 4 1 kDa Structural analysis revealed α helical dominance 62% with stable disulfide linkages, enhancing thermal stability up to 85°C In vitro bioactivity assays showed significant antioxidant capacity DPPH radical scavenging: 78 4 ± 3 1% and immunomodulatory effects IL 6 suppression: 41 2% in macrophage models Application trials in model dairy matrices yogurt and cheese analogs indicated improved shelf life extension by 18 days and enhanced probiotic viability under simulated gastric conditions The integration of these bio polypeptides into PharmaFood systems exhibited dual functionality: nutritional enrichment with essential amino acid profiles and therapeutic modulation via anti inflammatory pathways Stability under pasteurization 72°C, 15 s was retained at 94%, supporting industrial scalability Comparative analysis with synthetic peptides highlighted superior biocompatibility and lower immunogenicity This work establishes a circular bioeconomy model wherein dairy waste is valorized into high value biomolecular catalysts for next generation functional foods The findings underscore the transformative potential of nematode derived nanostructures in sustainable biomanufacturing, paving the way for eco efficient PharmaFood innovations with minimal environmental footprint Future directions include in vivo efficacy studies and pilot scale production optimization to bridge laboratory scale synthesis with commercial viability
کلیدواژهها
نویسندگان
Morteza Jamshid Eini
PhD in Food Technology, Islamic Azad University, North Tehran Branch, Tehran, Iran
Behzad Beizaei
PhD student in Food Technology, Islamic Azad University, Tehran Azad Medical Sciences Branch, Tehran, Iran
Samira Pirouzi
Ph D Student in Food Science and Technology, Department of nutrition and Food Science, Faculty of Food science, Islamic Azad University, mamagan, tabriz, Iran
Pegah Behpour
PhD student in Food Technology, Islamic Azad University, Tehran Science and Research Branch, Tehran, Iran
شیوه ارجاع
Jamshid Eini, Morteza and Beizaei, Behzad and Pirouzi, Samira and Behpour, Pegah,1404,Evaluation of the Performance of Nanostructured Proteins from Micro-Nematodes Extracted from Dairy Silage Fermentation Waste on the Synthesis of Novel Bio-Polypeptides and Their Applications in PharmaFood,The first national conference environment, smart agriculture, food security,Varamin
ارائهشده در
مجموعه مقالات اولین همایش ملی محیط زیست، کشاورزی هوشمند، امنیت غذایی28 آبان 1404 · ورامین